TESTING AND VALIDATION OF A NEW METHOD FOR THE INVESTIGATION OF FUNCTIONAL CHANGES IN HUMAN HEART AND SKELETAL MUSCLES
TESTING AND VALIDATION OF A NEW METHOD FOR THE INVESTIGATION OF FUNCTIONAL CHANGES IN HUMAN HEART AND SKELETAL MUSCLES (Abstract): Aim: Analysis of the efficiency of the use of permeabilized fiber in clinical medicine. Material and methods: Mitochondrial respiration was performed in 680 patients with dilated cardiomyopathy (DCM), hypertrophic cardiomyopathy (HCM), inflammatory cardiomyopathy (ICM) and ischemic heart disease/Ischemic cardiomyopathy (IHD/Ischemic CM) with heart failure (HF) 0-IY NYHA, as well as in the deltoid muscle during fitness in athletes. Subendomycocardial and deltoid muscle biopsy, myocardial scintigraphy, quantitative immunoblotting and electrophoresis were performed. Results: Scintigraphy revealed left ventricular (LV) perfusion defects of 85.6% in Ischemic CM/IHD and 81% in DCM. The indices of myocardial hypertrophy showed a 42% increase in tubulin and 33% increase in desmin levels, and of skeletal myosin beta isoforms by 20%. SM22 was increased 1.5-fold, Kinase Related protein KRP by 52%, Myosin Light Chain Kinase (MLCK) by 70%. Nuclei were bizarre and oversized. CrKmit fraction - 12.8, MB - 27.24, in the intact myocardium, 21.08 and 19.7, respectively. Mitochondrial respiration, Vmax / Vo 2.27 ± 0.17 1.52 ± 0.08 <0.001. Tissue respiration of the deltoid muscle in athletes correlated with the amount and density of mitochondria and was related to the level of physical fitness. Conclusions: The study shows that the use of this method of approaching and interpreting the data obtained through the permeabilized cell technique, largely based on personal clinical results, makes it possible to identify the earliest mitochondrial functional changes and their consequences on the energy balance and mechanical function of the heart, thus validating the method as a reference in the investigation of various cardiac diseases and assessment of skeletal tissue capacity related to fitness level.
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