HISTOLOGIC AND IMMUNOHISTOCHEMICAL ASSESSMENT OF GINGIVAL TISSUE’S CHANGES INDUCED BY PERIODONTAL DISEASE IN ASSOCIATION WITH CHRONIC HEPATITIS C
Periodontal disease and chronic hepatitis C are two types of chronic inflammatory reactions that target the supporting tissues of the teeth and the liver tissue, respectively. Common pathogenic pathways may be shared by the two types of disease, which the current study aims to histologically assess within the gingival tissue. Material and methods: The study comprised of gingival tissue samples, originating from periodontal patients with asymptomatic chronic hepatitis C and from periodontal patients with no systemic illness (collected during periodontal surgery), as well as from healthy control subjects (collected during extraction of maxillary premolars for orthodontic reasons). Upon standard histological preparation, part the samples were stained with hematoxylin-eosin technique and part were subjected to immunohistochemistry reaction for matrix-metalloproteinase-9 (MMP9) detection. Results revealed inflammatory morphopathological changes, which were significantly more intense and more frequent within the tissue samples of periodontal patients with chronic hepatitis C, than in those of systemically healthy ones with periodontal disease. The coloring reaction, used to evaluate the existence of MMP9 within the tissue samples, was significantly more positive for the group of periodontal and chronic hepatitis C patients than for the group of periodontal patients with no systemic illness. Conclusions: the inflammatory reaction is more intensive in the gingival tissue of periodontal patients with chronic hepatitis C than in that of periodontal patients without systemic conditions, proving the additional negative impact that the viral hepatic disease has on the inflammatory status, which periodontal disease involves.
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