HISTOLOGIC AND IMMUNOHISTOCHEMICAL ASSESSMENT OF GINGIVAL TISSUE’S CHANGES INDUCED BY PERIODONTAL DISEASE IN ASSOCIATION WITH CHRONIC HEPATITIS C

  • D.N. GHEORGHE University of Medicine and Pharmacy of Craiova
  • A. CAMEN University of Medicine and Pharmacy of Craiova
  • Liliana FOIA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Sorina SOLOMON “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Vasilica TOMA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Olivia Garofita MATEESCU University of Medicine and Pharmacy of Craiova
  • Amelia SURDU “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • I. ROGOVEANU University of Medicine and Pharmacy of Craiova
  • Petra SURLIN University of Medicine and Pharmacy of Craiova

Abstract

Periodontal disease and chronic hepatitis C are two types of chronic inflammatory reactions that target the supporting tissues of the teeth and the liver tissue, respectively. Common pathogenic pathways may be shared by the two types of disease, which the current study aims to histologically assess within the gingival tissue. Material and methods: The study comprised of gingival tissue samples, originating from periodontal patients with asymptomatic chronic hepatitis C and from periodontal patients with no systemic illness (collected during periodontal surgery), as well as from healthy control subjects (collected during extraction of maxillary premolars for orthodontic reasons). Upon standard histological preparation, part the samples were stained with hematoxylin-eosin technique and part were subjected to immunohistochemistry reaction for matrix-metalloproteinase-9 (MMP9) detection. Results revealed inflammatory morphopathological changes, which were significantly more intense and more frequent within the tissue samples of periodontal patients with chronic hepatitis C, than in those of systemically healthy ones with periodontal disease. The coloring reaction, used to evaluate the existence of MMP9 within the tissue samples, was significantly more positive for the group of periodontal and chronic hepatitis C patients than for the group of periodontal patients with no systemic illness. Conclusions: the inflammatory reaction is more intensive in the gingival tissue of periodontal patients with chronic hepatitis C than in that of periodontal patients without systemic conditions, proving the additional negative impact that the viral hepatic disease has on the inflammatory status, which periodontal disease involves.

Author Biographies

D.N. GHEORGHE, University of Medicine and Pharmacy of Craiova

Faculty of Dental Medicine
Department of Periodontology

A. CAMEN, University of Medicine and Pharmacy of Craiova

Faculty of Dental Medicine
Department of Oral Surgery

Liliana FOIA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Surgery

Sorina SOLOMON, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Odontology and Periodontology

Vasilica TOMA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Surgery

Olivia Garofita MATEESCU, University of Medicine and Pharmacy of Craiova

Faculty of Medicine
Department of Histology

Amelia SURDU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Odontology and Periodontology

I. ROGOVEANU, University of Medicine and Pharmacy of Craiova

Faculty of Medicine
Department of Gastroenterology

Petra SURLIN, University of Medicine and Pharmacy of Craiova

Faculty of Dental Medicine
Department of Periodontology

References

1. Offenbacher S, Barros SP, Beck, JD. Rethinking periodontal inflammation. J Periodontol 2008; 79(8S): 1577-1584.
2. Olteanu M, Surlin P, Oprea B, Rauten AM, Popescu RM, Niţu M, et al. Gingival inflammatory infil-trate analysis in patients with chronic periodontitis and diabetes mellitus. Rom J Morphol Embryol 2011; 52(4): 1311-1317.
3. Trombelli L, Tatakis DN, Scapoli C, Bottega S, Orlandini E, Tosi M. Modulation of clinical expression of plaque-induced gingivitis. J Clin Periodontol 2004; 31(4): 239-252.
4. Page RC, Schroeder HE. Pathogenesis of inflammatory periodontal disease. A summary of current work. Lab Invest 1976; 34(3): 235-249.
5. Armitage GC, Cullinan MP, Seymour GJ. Comparative biology of chronic and aggressive periodontitis: introduction. Periodontol 2000 2010; 53(1): 7-11.
6. Kornman KS. Mapping the pathogenesis of periodontitis: a new look. J Periodontol 2008; 79(8S): 1560-1568.
7. Signat B, Roques C, Poulet P, Duffaut D. Role of Fusobacterium nucleatum in periodontal health and disease. Curr Issues Mol Biol 2011; 13(2): 25-36.
8. Nanci A, Bosshardt DD. Structure of periodontal tissues in health and disease. Periodontol 2000 2006; 40(1): 11-28.
9. Steinberg BE, Grinstein S. Unconventional roles of the NADPH oxidase: signaling, ion homeostasis, and cell death. Sci. STKE 2007; 379:11.
10. Souto GR, Queiroz Jr CM, Costa FO, Mesquita RA. Relationship between chemokines and dendritic cells in human chronic periodontitis. J Periodontol 2014; 85(10):1416-1423.
11. Manicone AM, McGuire JK. Matrix metalloproteinases as modulators of inflammation. Semin Cell Dev Biol 2008; 19(1): 34-41.
12. Sorsa T, Tjäderhane L, Salo T. Matrix metalloproteinases (MMPs) in oral diseases. Oral Dis 2004; 10(6): 311-318.
13. Şurlin P, Rauten AM, Pirici D, Oprea B, Mogoantă L, & Camen A. Collagen IV and MMP-9 expres-sion in hypertrophic gingiva during orthodontic treatment. Rom J Morphol Embryol 2012; 53(1): 161-165.
14. Kim KA, Chung SB, Hawng EY, Noh SH, Song KH, Kim HH, et al. Correlation of expression and activity of matrix metalloproteinase-9 and-2 in human gingival cells of periodontitis patients. J Perio-dontal Implant Sci 2013; 43(1): 24-29.
15. Sapna G, Gokul S, Bagri-Manjrekar K. Matrix metalloproteinases and periodontal diseases. Oral Dis 2014; 20(6): 538-550.
16. Surlin P, Oprea B, Solomon SM, Popa SG, Moţa M, Mateescu GO, et al. Matrix metalloproteinase -7, -8, -9 and-13 in gingival tissue of patients with type 1 diabetes and periodontitis. Rom J Morphol Em-bryol 2014; 55(3S): 1137-1141.
17. Özdemir SP, Kurtiş B, Tüter G, Bozkurt Ş, Gültekin SE, Sengüven B, et al. Effects of low-dose doxycycline and bisphosphonate clodronate on alveolar bone loss and gingival levels of matrix metal-loproteinase-9 and interleukin-1β in rats with diabetes: a histo-morphometric and immunohistochemical study. J Periodontol 2012; 83(9):1172-1182.
18. Siloşi I, Boldeanu MV, Cojocaru M, Biciuşcă V, Pădureanu V, Bogdan M et al. The relationship of cytokines IL-13 and IL-17 with autoantibodies profile in early rheumatoid arthritis. J Immunol Res, 2016; Article ID 3109135, 10 pages.
19. Alter MJ. Epidemiology of hepatitis C virus infection. World J Gastroenterol 2007; 13(17): 2436.
20. Denniston MM, Jiles RB, Drobeniuc J, Klevens RM, Ward JW, McQuillan GM, et al. Chronic hepati-tis C virus infection in the United States, national health and nutrition examination survey 2003 to 2010. Ann Intern Med 2014; 160(5): 293-300.
21. Gheorghe DN, Foia L, Toma V, Surdu A, Herascu E, Popescu DM, et al. Hepatitis C Infection and Periodontal Disease: Is there a Common Immunological Link? J Immunol Res 2018; Article ID 8720101, 9 pages.
22. Moskow BS, Poison AM. Histologic studies on the extension of the inflammatory infiltrate in human periodontitis. J Clin Periodontol 1991; 18(7): 534-542.
23. Lorenzi T, Niţulescu EA, Zizzi A, Lorenzi M, Paolinelli F, Aspriello SD, et al. The novel role of HtrA1 in gingivitis, chronic and aggressive periodontitis. PloS one 2014; 9(6): e96978.
24. Andreescu CF, Mihai LL, Răescu M, Tuculină MJ, Cumpătă CN, Ghergic DL. Age influence on periodontal tissues: a histological study. Rom J Morphol Embryol 2013; 54(3S): 811-815.
25. Bosch FX, Ouhayoun JP, Bader BL, Collin C, Grund C, Lee I, Franke WW. Extensive changes in cytokeratin expression patterns in pathologically affected human gingiva. Virchows Archiv B 1989; 58(1): 59.
26. Smith M, Seymour GJ, Cullinan MP. Histopathological features of chronic and aggressive periodonti-tis. Periodontol 2000 2010; 53(1): 45-54.
27. Holmstrup P. Histopathology of periodontal diseases. In: Wilson TW, Kornman KS editors. Funda-mentals of Periodontics. Quintessence Publishing Co, Inc, 2003, 39-46.
28. Ertugrul AS, Dursun R, Dundar N, Avunduk MC, Hakki SS. MMP-1, MMP-9, and TIMP-1 levels in oral lichen planus patients with gingivitis or periodontitis. Arch Oral Biol 2013; 58(7): 843-852.
29. Surlin P, Silosi I, Rauten AM, Cojocaru M, Foia L. Involvement of TSP1 and MMP9/NGAL in angi-ogenesis during orthodontic periodontal remodeling. Scientific World Journal 2014, Article ID 421029, 6 pages.
30. Lazăr L, Loghin A, Bud E, Cerghizan D, Horváth E, Nagy EE. Cyclooxygenase-2 and matrix metal-loproteinase-9 expressions correlate with tissue inflammation degree in periodontal disease. Rom J Morphol Embryol 2015; 56(4): 1441-1446.
31. Akca G, Tuncbilek S, Sepici-Dincel A. Association between matrix metalloproteinase (MMP)-2, MMP-9 and total antioxidant status of patients with asymptomatic hepatitis C virus infection. Lett Appl Microbiol 2013; 57(5): 436-442.
32. El Samanoudy A, Monir R, Badawy A, Ibrahim L, Farag K, El Baz S, et al. Matrix metalloproteinase-9 gene polymorphism in hepatocellular carcinoma patients with hepatitis B and C viruses. Genet Mol Res 2014; 13(3): 8025-8034.
33. Saftoiu A, Ciurea T, Georgescu C, Banita M, Comanescu V, Rogoveanu I, et al. Immunohistochemical assessment of proliferating cell nuclear antigen in primary hepatocellular carcinoma and dysplastic nodules. J Cell Mol Med 2013; 7(4): 436-446.
34. Reif S, Somech R, Brazovski E, Reich R, Belson A, Konikoff FM, Kessler A. Matrix metalloprotein-ases 2 and 9 are markers of inflammation but not of the degree of fibrosis in chronic hepatitis C. Di-gestion 2005; 71(2): 124-130.
35. Albu E, Lupascu D, Filip C, Zamosteanu N, Jaba I, Linic IS, Sosa I. Hyperhomocysteinemia is an indicator of oxidative stress. Med Hypoth 2012; 78(4): 554-555.
36. Sandulescu L, Rogoveanu I, Gheonea IA, Cazacu S, Saftoiu A. Real-time elastography applications in liver pathology between expectations and results. J Gastrointestin Liver Dis 2013; 22(2): 221-227.
37. Marinosci F, Bergamini C, Fransvea E, Napoli N, Maurel P, Dentico P, et al. Clinical Role of Serum and Tissue Matrix Metalloprotease-9 Expression in Chronic HCV Patients Treated with Pegylated IFN-α2b and Ribavirin. J Interferon Cytokine Res 2005; 25(8): 453-458.
Published
2018-12-27