NEW ORIGINAL IN VITRO METHOD TO ASSESS CHOLINESTERASE REACTIVITY IN ORGANOPHOSPHATE POISONING

  • L. SORODOC ”Gr.T. Popa” University of Medicine and Pharmacy Iași
  • Catalina LEONTE ”Gr.T. Popa” University of Medicine and Pharmacy Iași
  • Ema LARGU ”Sf. Ioan” Emergency Clinic Hospital Iași
  • O. PETRIS ”Sf. Ioan” Emergency Clinic Hospital Iași
Keywords: ORGANOPHOSPHATE, CHOLINESTERASE, TOXOGONIN

Abstract

Assessment of organophosphate poisoning
could benefit from a safe, non-expensive, easy to perform, quick (< 1 hour) test,
which evaluates the level of cholinesterase activity in vitro regarding to the capability of
oximes to reactivate OF-blocked cholinesterase. In the proposed protocol, 0.5 mL of sample
serum is incubated, prior to the evaluation of level of cholinesterase activity, with 5 mL of a
Toxogonin® dilution (0.125 mg) for 30 minutes at 37°C. For the standardization of the newly
proposed protocol, several important issues were documented in the present article. The new
original method of assessing cholinesterase reactivability will consist in an advantage for the
diagnosis, prognostic evaluation and therapeutic orientation in OF intoxication.

Author Biographies

L. SORODOC, ”Gr.T. Popa” University of Medicine and Pharmacy Iași

School of Medicine
Internal Medicine Department

”Sf. Ioan” Emergency Clinic Hospital Iași
Medical Clinic

Catalina LEONTE, ”Gr.T. Popa” University of Medicine and Pharmacy Iași

School of Medicine
Internal Medicine Department

”Sf. Ioan” Emergency Clinic Hospital Iași
Medical Clinic

Ema LARGU, ”Sf. Ioan” Emergency Clinic Hospital Iași

Biochemistry and Toxicology Laboratory

O. PETRIS, ”Sf. Ioan” Emergency Clinic Hospital Iași

Medical Clinic

”Gr.T. Popa” University of Medicine and Pharmacy Iași
School of Medicine

Nursing Department

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Published
2019-10-21
Section
INTERNAL MEDICINE - PEDIATRICS