EVALUAREA EXPRESIEI PROTEINELOR P16INK4A ȘI Ki-67 ÎN NEOPLAZIA INTRAEPITELIALÃ CERVICALÃ ȘI CORELAREA ACESTORA CU INFECȚIA HPV-HR

  • Carmen UNGUREANU Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași
  • S. TELEMAN Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași
  • Demetra SOCOLOV Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași
  • Gabriela ANTON Institutul de Virusologie ”Șt. S. Nicolau” București
  • Maria - Sultana MIHAILOVICI Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași
Keywords: P16INK4A, KI-67, CERVICAL INTRAEPITHELIAL NEOPLASIA (CIN), HIGH RISK HUMAN PAPILLOMA VIRUS (HPV-HR)

Abstract

Objective: The aim of our study was to evaluate the expression of p16INK4A and
Ki-67 in cervical intraepithelial neoplasia (CIN) and to correlate the high-risk human papillomavirus
(HPV) infection with these two biomarkers. Material and method: Sixty patients were
included in our study. All of the patient underwent colposcopy-guided biopsy to asses the grade
of CIN. For the immunohistochemistry exam we used p16INK4A and Ki-67 antibody and their
expression was semiquantitatively classified in 4 classes: 0, 1, 2 and 3. HPV genotyping was
used to detect high-risk HPV. Results: 7 of our cases were benign (11.66%); 18 cases
presented CIN1 (30%), 14 cases CIN2 (23.33%), 16 cases CIN3 (26.66%) and 5 cases
invasive squamous cell carcinoma (8.33%). We observed that the expression of p16INK4A and
Ki-67 were positively associated with CIN grade. p16INK4A expressions increased significantly
with high-risk HPV infection. We observed a positive correlation between the expression of the
p16INK4A and Ki-67. Conclusion: In our study, the expression of p16 and Ki-67 were
positively related to the CIN grade. p16INK4A expressions of high-risk HPV specimens significantly
increased more than Ki-67. Therefore, in the diagnosis of CIN and high-risk HPV
infection, p16INK4A can be a useful biomarker.

Author Biographies

Carmen UNGUREANU, Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași

Facultatea de Medicinã
Disciplina de Morfopatologie

S. TELEMAN, Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași

Facultatea de Medicinã
Disciplina de Morfopatologie

Demetra SOCOLOV, Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași

Facultatea de Medicinã

Departamentul de Obstetricã-Ginecologie

Gabriela ANTON, Institutul de Virusologie ”Șt. S. Nicolau” București

 

 

Maria - Sultana MIHAILOVICI, Universitatea de Medicină și Farmacie ”Gr. T. Popa” Iași

Facultatea de Medicinã
Disciplina de Morfopatologie

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Published
2019-11-12