EVALUAREA EXPRESIEI PROTEINELOR P16INK4A ȘI Ki-67 ÎN NEOPLAZIA INTRAEPITELIALÃ CERVICALÃ ȘI CORELAREA ACESTORA CU INFECȚIA HPV-HR
Objective: The aim of our study was to evaluate the expression of p16INK4A and
Ki-67 in cervical intraepithelial neoplasia (CIN) and to correlate the high-risk human papillomavirus
(HPV) infection with these two biomarkers. Material and method: Sixty patients were
included in our study. All of the patient underwent colposcopy-guided biopsy to asses the grade
of CIN. For the immunohistochemistry exam we used p16INK4A and Ki-67 antibody and their
expression was semiquantitatively classified in 4 classes: 0, 1, 2 and 3. HPV genotyping was
used to detect high-risk HPV. Results: 7 of our cases were benign (11.66%); 18 cases
presented CIN1 (30%), 14 cases CIN2 (23.33%), 16 cases CIN3 (26.66%) and 5 cases
invasive squamous cell carcinoma (8.33%). We observed that the expression of p16INK4A and
Ki-67 were positively associated with CIN grade. p16INK4A expressions increased significantly
with high-risk HPV infection. We observed a positive correlation between the expression of the
p16INK4A and Ki-67. Conclusion: In our study, the expression of p16 and Ki-67 were
positively related to the CIN grade. p16INK4A expressions of high-risk HPV specimens significantly
increased more than Ki-67. Therefore, in the diagnosis of CIN and high-risk HPV
infection, p16INK4A can be a useful biomarker.
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