MEASUREMENT OF TOTAL ANTIOXIDANT ACTIVITY WITH CHLORPROMAZINE RADICAL CATION

  • Alina Monica MIFTODE ”Gr.T. Popa” University of Medicine and Pharmacy Iași
  • Alina STEFANACHE ”Gr.T. Popa” University of Medicine and Pharmacy Iași
  • Maria STAN ”Gr.T. Popa” University of Medicine and Pharmacy Iași
  • V. DORNEANU ”Gr.T. Popa” University of Medicine and Pharmacy Iași
Keywords: CHLORPROMAZINE RADICAL CATION, ANTIOXIDANT ACTIVITY, SPECTROPHOTOMETRIC METHODS

Abstract

In the present study there is described a simple and sensitive
method for the evaluation of antioxidant activity in chlorpromazine-Fe (III) system using
ascorbic acid as standard. Material and method: Chlorpromazine [2-chloro-N-(3-dimethylaminopropyl)-
phenothiazine] is oxidized in acidic media by Fe (III) with the formation of a
stable radical cation characterized by an intense visible absorbtion band (l = 540 nm). The
optimum parameters for the stability of the radical cation have been studied: molar ratio
chlorpromazine-Fe (III), pH, solvents. Spectrophotometric methods have been used in these
studies. Results: The chlorpromazine radical cation is stable in the acidic media (pH = 3) at
a molar ratio chlorpromazine:Fe (III) of 2:1. Ascorbic acid reduces these radicals and induces
a decrease of absorbance. Percent inhibition was calculated and plotted as a function of the
concentration of standard antioxidant solutions. The results show that percent inhibition varies
in a linear manner with the ascorbic acid concentration. Percent inhibition is higher when the
antioxidant solution is added after generation of radical cation. Conclusions: It has been
developed a method for evaluating antioxidant activity in the chlorpromazine:Fe (III) system
using ascorbic acid as a standard. The method is fast, simple and sensitive; it can be applied
for the detection and evaluation of the antioxidant activity of simple or complex systems.

Author Biographies

Alina Monica MIFTODE, ”Gr.T. Popa” University of Medicine and Pharmacy Iași

School of Pharmacy
Department of General and Inorganic Chemistry

Alina STEFANACHE, ”Gr.T. Popa” University of Medicine and Pharmacy Iași

School of Pharmacy
Department of General and Inorganic Chemistry

Maria STAN, ”Gr.T. Popa” University of Medicine and Pharmacy Iași

School of Pharmacy

Department of Analytical Chemistry

V. DORNEANU, ”Gr.T. Popa” University of Medicine and Pharmacy Iași

School of Pharmacy

Department of Analytical Chemistry

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Published
2019-11-14