A RAPID, SIMPLE AND SENSITIVE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF NO FROM NO DONORS

  • A. SAVA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Alina PANAINTE “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Nela BIBIRE “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Sandra CONSTANTIN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • A. FOCSA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • F. BURON University of Orleans
  • S. ROUTIER University of Orleans
  • Lenuta PROFIRE “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Abstract

Aim. To develop and validate a spectrophotometric UV-VIS method for the determination of nitric oxide (NO) released from NO donors, such as nitric oxide-releasing nonsteroidal anti-inflammatory drugs (NO-NSAIDs), with poor solubility in aqueous medium. Material and methods: The method is based on the nitric oxide-mediated stress (nitrosative stress) through sulfanilamide that reacts with N-(1-naphthyl)ethylenediamine dihydrochloride to form a pink-red azo dye with maximum peak at 540nm. The method was validated in term of linearity, detection limit, quantification limit, precision, and accuracy. Results: The linearity of the method was proved for range of 0.78-100μM NaNO2 with a correlation coefficient of 0.9998. The accuracy of the method was checked by recovery experiment performed at three concentrations, i.e., 20 μM, 40 μM and 60μM NaNO2 and the recovery (%) was found to be in the range of 98.34% and 101.96%. The low values of relative standard deviation (RSD) are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as intraday and interday variations. An RSD less than 2% indicates that the method is precise. Conclusions: This research offers all the proofs that recommend the use of this method for the determination of NO released from NO donors, such as NO-NSAID, using a hydroalcoholic medium, as it has good sensitivity, precision, and accuracy.

Author Biographies

A. SAVA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Pharmacy
Department of Analytical Chemistry

Alina PANAINTE, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Pharmacy
Department of Analytical Chemistry

Nela BIBIRE, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Pharmacy
Department of Analytical Chemistry

Sandra CONSTANTIN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Pharmacy
Department of Pharmaceutical Chemistry

A. FOCSA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Pharmacy
Department of Pharmaceutical Chemistry

F. BURON, University of Orleans

Institut de Chimie Organique et Analytique, CNRS, UMR 7311, France

S. ROUTIER, University of Orleans

Institut de Chimie Organique et Analytique, CNRS, UMR 7311, France

Lenuta PROFIRE, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Pharmacy
Department of Pharmaceutical Chemistry

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Published
2020-12-23