THE PROLONGATION OF THE ANTINOCICEPTIVE EFFECTS OF NOVEL NANOSYSTEMS ENTRAPPING DICLOFENAC SODIUM  IN SOMATIC PAIN IN RATS

Ana-Maria Raluca PAUNA; C. I. STAN; Liliana MITITELU-TARTAU

Ana-Maria Raluca PAUNA “Grigore T. Popa” University of Medicine and Pharmacy, Iasi
C. I. STAN “Grigore T. Popa” University of Medicine and Pharmacy, Iasi
Liliana MITITELU-TARTAU “Grigore T. Popa” University of Medicine and Pharmacy, Iasi

Keywords: LIPID VESICLES, DICLOFENAC SODIUM, TAIL FLICK, NOCICEPTION, RATS

Abstract

The prolongation of the antinociceptive effects of novel nanosystems entrapping diclofenac sodium in somatic pain in rats (Abstract): The aim of this study was the investigation effects of nanosystems incorporating diclofenac sodium on the nociceptive reactivity in rats. Material and methods: In the experimental study were used 24 white healthy, non-genetically modified Wister rats, weighing 200-250 g, with a uniform distribution by sex, purchased from the National Medical-Military Institute “Cantacuzino” for Research and Development, Băneasa, Bucharest. The substances were given orally as follows: Group I (control): distilled water 0.3 mL/100 g body weight, Group 2 (DCF): 5 mg/kg body weight (kbw) diclofenac sodium; Group 3 (DCF-ves): lipid vesicles entrapping diclofenac sodium 5 mg/kbw. The experimental protocol was approved by the University Committee for Research and Ethical Issues, in compliance with the international ethical guidelines regarding the handling of laboratory animals. Data were statistically processed using SPSS software for Windows 17.0 version and ANOVA one-way method. Results: Lipid vesicles containing diclofenac, stabilized with chitosan were designed. The measurements indicated a mean size of 562 nm and a mean Zeta potential of +45 mV, thus suggest a reasonable stability of the colloidal suspension. The use of DCF resulted in a prolongation of the latency period response to thermal noxious tail stimulation, with a statistical significance during 90 minutes in the experiment. The treatment with DCF-ves was accompanied by a substantial increase of the latency time reaction in the interval between 2 hours and 8 hours after the administration. Conclusions: Both entrapped and non-entrapped DCF proved to have an important analgesic effect in tail flick test in rats. The administration of lipid vesicles as carriers for DCF demonstrated a prolonged antinociceptive activity, compared to the non-entrapped drug in this cutaneous pain model in rats.

Author Biographies

Ana-Maria Raluca PAUNA, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi

Ph.D. student
Faculty of Medicine
Department of Morpho-functional Sciences (II)

C. I. STAN, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi

Department of Morpho-functional Sciences (I)

Liliana MITITELU-TARTAU, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi

Department of Morpho-functional Sciences (II)

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