THE INFLUENCE OF SYNTHESIS TEMPERATURE ON A POLYURETHANE CARRIER USED FOR THE TRANSFER OF 2’-DEOXYCYTIDYLIC ACID

  • Roxana Maria JELERIU Victor Babes” University of Medicine and Pharmacy Timisoara
  • Lidia Manuela ONOFREI “Victor Babes” University of Medicine and Pharmacy Timisoara
  • F. BORCAN “Victor Babes” University of Medicine and Pharmacy Timisoara
  • Ramona Carmen ALBUILESCU “Victor Babes” University of Medicine and Pharmacy Timisoara
  • Maria PUIU “Victor Babes” University of Medicine and Pharmacy Timisoara

Abstract

2’-Deoxycytidylic acid, a nucleotide based on cytosine, deoxyribose and phosphate group, is a fundamental unit of nucleic acids. Various supplements containing nucleosides or nucleotides have been formulated for patients with genetic defects resulting in the presence of incorrect nucleotides. The aims of the current research were to obtain and to characterize polyurethane transporters that can be used for the transfer of nucleotides through body membranes. Materials and methods: Three samples have been developed based on different synthesis temperatures and the effects of this parameters on the properties of the final products were assessed; there were measured the solubility, pH, refractivity index, the encapsulation efficacy and release profile, size distribution and surface charge, cells proliferation and irritation tests on mice skin. Results: The obtained mean values show that were obtained almost neutral acido-basic particles with sizes range from 126 to 814 nm; Zeta potential values indicate the tendency to form clusters in samples with synthesis temperatures above 40°C, while UV-Vis evaluations show an approx. 60% encapsulation efficacy. Regarding the biosafety profile of the samples, the assays on cells culture and mice skin did not elicit important side effects at tested concentration. Conclusions: The findings of this preclinical study suggest that polyurethane carriers exhibit promise as a therapeutic or preventative measure for a range of diseases by addressing genetic irregularities, demonstrating their safety for such applications.

Author Biographies

Roxana Maria JELERIU, Victor Babes” University of Medicine and Pharmacy Timisoara

Faculty of Medicine
Ph.D. School

Lidia Manuela ONOFREI, “Victor Babes” University of Medicine and Pharmacy Timisoara

Faculty of Medicine
Ph.D. School

F. BORCAN, “Victor Babes” University of Medicine and Pharmacy Timisoara

Faculty of Pharmacy
Department I (Advanced Instrumental Screening Center)

Ramona Carmen ALBUILESCU, “Victor Babes” University of Medicine and Pharmacy Timisoara

Faculty of Medicine
Department XI (Pediatrics II)

Maria PUIU, “Victor Babes” University of Medicine and Pharmacy Timisoara

Faculty of Medicine
Department II (Microscopic Morphology)
Regional Center of Medical Genetics

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Published
2024-03-29