THE BENEFIT OF MOLECULAR MICROBIOLOGICAL ASSAYS IN OPTIMAL MONITORING OF PERIODONTAL DISEASE. A RETROSPECTIVE STUDY

  • G. NICHITEAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • I. LUCHIAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Monica Silvia TATARCIUC “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Oana BEJAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Simona GIUSCA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Ancuta GORIUC “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • C. DAMIAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Irina-Draga CARUNTU “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Ramona Gabriela URSU “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Abstract

Periodontal disease is among the most frequent pathologies worldwide, with multiple consequences to the quality of daily patient life. Aim: To assess the prevalence and the DNA load of etiologic agents in selected patients with periodontal disease. Materials and methods: 55 saliva samples from periodontal disease patients were analyzed by multiplex Real Time PCR, using specific primers for: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola. The positive sampled were further analysed for the evaluation the antibiotic susceptibility to Clindamycin, Metronidazole, Amoxicillin, Doxycycline, Ciprofloxacin, and Clarithromycin. Results: The most frequent bacteria detected were Treponema denticola, in samples from 47 patients (85.5%), followed by Porphyromonas gingivalis, in 43 patients (78.2%) and Aggregatibacter actinomycetemcomitans, in 5 patients (9.1%). Besides these 3 periodontal pathogens, one patient was positive for Tannerella forsythia, Prevotella intermedia, Eubacterium nodatum and Capnocytophaga gingivalis. Conclusions: The microbiologic diagnosis using sensitive molecular assay is compulsory for modern and optimal periodontal diseases assessment.

Author Biographies

G. NICHITEAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Dental Technology

I. LUCHIAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Periodontology

Monica Silvia TATARCIUC, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Dental Technology

Oana BEJAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Orthodontics

Simona GIUSCA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Department of Morpho-functional Sciences (I)

Ancuta GORIUC, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Dental Medicine
Department of Biochemistry

C. DAMIAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Department of Preventive Medicine and Interdisciplinarity

Irina-Draga CARUNTU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Department of Morpho-functional Sciences (I)

Ramona Gabriela URSU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Department of Preventive Medicine and Interdisciplinarity

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Published
2024-03-29