THE DEVELOPMENT OF ALGINATE-BASED MATRICES LOADED WITH RIFAMPICIN FOR WOUND HEALING

  • T. BIBIRE “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Cristina Mihaela GHICIUC “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Cătălina Natalia YILMAZ “Dokuz Eylül” University, Izmir, Turkey
  • Ramona Gabriela URSU “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • R. DANILA “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Abstract

The proposed study aims to develop polymeric systems based on alginate and N-vinylcaprolactam (NVCL) coated with poly(D,L-lactide-co-glycolide) (PLGA) as vehicles for Rifampicin (RIF) as active substance. Rifampicin is an agent used for tuberculosis treatment, but the present study proposes to be used for wound healing. The proposed topic is a complex study comprising a lot of data; herein information on Rifampicin loaded matrix and its antimicrobial activity against the main strains of the following bacteria: S. aureus, MRSA, E. coli, P. aeruginosa. Materials and methods: Alginate and NVCL were used as main materials for polymer preparation then coated with PLGA; the polymeric matrix was loaded with Rifampicin as active substance. The preparation of polymer consisted of various steps such as polymerization technique, water in oil in water emulsification and then matrix lyophilization for characterization in solid state. Chromatography was used to indicate the presence of the drug (TLC) and then HPLC was used to quantify the amount of it. The antimicrobial activity was tested by culturing the tested substances against some bacteria e.g. S. aureus, MRSA, E. coli, P. aeruginosa. Results: The tested matrix showed positive results in terms of drug loading and antimicrobial activity and will be further investigated for IC50 and cytotoxicity. Conclusions: Based on the obtained results, the proposed rifampicin-loaded alginate-based matrix shows potential in wound treatment, including surgical wound care.

Author Biographies

T. BIBIRE, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Ph.D. Student
“Sf. Spiridon” County Clinical Emergency Hospital, Iasi, Romania

Cristina Mihaela GHICIUC, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Department of Morpho-functional Sciences (II)
“Sf. Maria” Clinical Hospital for Children, Iasi, Romania

Cătălina Natalia YILMAZ, “Dokuz Eylül” University, Izmir, Turkey

Faculty of Science / Department of Chemistry, Biochemistry Division

Ramona Gabriela URSU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Department of Preventive Medicine and Interdisciplinarity

R. DANILA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Faculty of Medicine
Department of Surgery (I)
“Sf. Spiridon” County Clinical Emergency Hospital, Iasi, Romania

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Published
2024-03-29