METFORMIN’S IMPACT ON SERUM IMUNOGLOBULINS AND BRONCHOALVEOLAR LAVAGE CELLS IN MICE WITH COMPLICATED SEVERE ASTHMA

Authors

  • Diana Cezarina PETRESCU Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • A.G. VICOVAN Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Daniela CONSTANTINESCU Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Elena IFTIMI Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Mousa SHA’AT Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Carmen SOLCAN “Ion Ionescu de la Brad” University of Life Sciences of Iasi, Romania
  • Lacramioara OCHIUZ Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Cristina Mihaela GHICIUC Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Loredana Beatrice UNGUREANU Grigore T. Popa” University of Medicine and Pharmacy Iasi

DOI:

https://doi.org/10.22551/MSJ.2024.02.13

Abstract

Background: Severe asthma represents an important burden of mortality and morbidity in asthma being a clinical condition that has the risk of evolution to acute lung injury (ALI). Based on the anti-inflammatory and airway anti-remodeling effect of Metformin we presumed that it will promote beneficial effects in severe asthma complicated to ALI. Our study aimed to evaluate the effects of Metformin on a murine model of severe asthma complicated with ALI by assessing the modulatory effect of Metformin of plasma IgE levels and on differential cell count from bronchoalveolar lavage fluid. Materials and methods: There were three groups of BALB/c mice with ovalbumin (OVA)-induced asthma followed by ALI induction which consisted in OVA sensitization (days 0 and 7) and challenge (days 14, 15, 16 and 17) followed by lipopolysaccharide (LPS) intratracheal instillations (days 15 and 17) treated 30 min before OVA, in days 14-17: positive control group for disease (OVA+LPS), Dexamethasone treatment group (DEXA + OVA + LPS) and Metformin treatment group (OVA + LPS + METF). Measurement of OVA-specific IgE levels was made and bronchoalveolar lavage fluid (BALF) was collected in order to apply differential cell count. Results: administration of Metformin decreased the airway macrophage infiltration with no modulatory effect on airway neutrophilic infiltration. Conclusions: Metformin has the potential to influence the physio-pathological changes that occur in asthma followed by ALI. However, new studies to explore the specific mechanism by which Metformin influences the inflammatory and immune response in asthma/ALI overlap are needed.

Author Biographies

  • Diana Cezarina PETRESCU, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II)

  • A.G. VICOVAN, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II)

  • Daniela CONSTANTINESCU, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (I)

  • Elena IFTIMI, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (I)

  • Mousa SHA’AT, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Pharmacy
    Department of Pharmaceutical Technology

  • Carmen SOLCAN, “Ion Ionescu de la Brad” University of Life Sciences of Iasi, Romania

    Faculty of Veterinary Medicine / Department IX

  • Lacramioara OCHIUZ, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Pharmacy
    Department of Pharmaceutical Technology

  • Cristina Mihaela GHICIUC, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II)
    “Sf. Maria” Clinical Hospital for Children, Iasi, Romania

  • Loredana Beatrice UNGUREANU, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (I)

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Additional Files

Published

2024-06-28

Issue

Vol. 128 No. 2 (2024): The Medical-Surgical Journal

Section

BASIC SCIENCES

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