THE RELATIONSHIP BETWEEN SOIL QUALITY, SECONDARY METABOLITE CONTENT AND ANTIOXIDANT EFFICACY IN CARTHAMUS TINCTORIUS L. AND CALENDULA OFFICINALIS L.
DOI:
https://doi.org/10.22551/7gd8vm98Abstract
Asteraceae plants are commonly used for their antibacterial, anti-inflammatory and anti-aging effects. They are widely utilized in therapeutic and cosmetic formulations, while also serving decorative purposes. Safflower also serve a range of purposes, from medicine, food coloring and ornaments. Calendula officinalis is widely cultivated for medicinal and cosmetic purposes, especially for its therapeutic effects in topical formulations. Thus, the aim of our study was to investigate the influence of soil fertility on the secondary metabolites profile and its antioxidant activity. Materials and methods: To achieve this, Calendula officinalis and Carthamus tinctorius were cultivated in Romania under two different conditions: local soil, and enriched soil respectively. After collection, fragments of dried leaves and inflorescences were extracted with either ethanol or acetonitrile and analyzed. Semiquantitative analysis was performed using the HPLC method and he total phenolic content of each sample was quantified with Folin-Ciocalteu reagents. For the in vitro tests, 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) was used to evaluate the radical scavenger activity, and reaction with ferrozine was used to evaluate the ferrous iron chelation capacity. Results: The quantitative analyses showed a clear influence of soil fertility on polyphenolic profile, impacting the antioxidant activity. Conclusions: The timing of harvest and the soil quality should be taken into consideration in order to ensure optimal pharmacological effects. Keywords:
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