ATOMIC FORCE MICROSCOPY CHARACTERIZATION OF A THREE-DIMENSIONAL PORCINE COLLAGEN MATRIX FOR PERIODONTAL APPLICATIONS UNDER HYDRATED CONDITIONS

Authors

  • C. COJOCARU Grigore T. Popa University of Medicine and Pharmacy Iasi, Romania
  • D. I. VIRVESCU Grigore T. Popa University of Medicine and Pharmacy Iasi, Romania
  • Oana-Maria BUTNARU Grigore T. Popa University of Medicine and Pharmacy Iasi, Romania
  • Daniela ARGATU Grigore T. Popa University of Medicine and Pharmacy Iasi, Romania
  • F. C. BIDA Grigore T. Popa University of Medicine and Pharmacy Iasi, Romania
  • St. L. TOMA Gheorghe Asachi Technical University of Iasi, Romania
  • V CONSTANTIN Grigore T. Popa University of Medicine and Pharmacy Iasi, Romania
  • I. LUCHIAN Grigore T. Popa University of Medicine and Pharmacy Iasi, Romania

DOI:

https://doi.org/10.22551/MSJ.2026.02.20

Abstract

The aim of this study was to characterize the nanoscale surface topography of a three-dimensional porcine collagen matrix under hydrated conditions, simulating a physiologically relevant environment. Materials and methods: Atomic force microscopy (AFM) was performed in hydrated conditions to evaluate surface topography, deflection signal, three-dimensional reconstruction, surface profile, and quantitative roughness parameters. Results: The collagen matrix exhibited a continuous, moderately undulating surface without detectable structural defects. Topographic analysis revealed a homogeneous distribution of elevations and depressions, consistent with an isotropic collagen network. Deflection imaging enhanced the visualization of local slope variations, indicating microstructural heterogeneity. Three-dimensional reconstruction confirmed uniform spatial organization with gradual height transitions. Quantitative analysis demonstrated moderate roughness (Sa = 0.1179 µm; Sq = 0.1765 µm) and limited maximum height (Sz = 1.541 µm), suggesting the absence of pronounced irregularities. Height distribution showed a centered dispersion around the mean plane. Conclusions: The hydrated collagen matrix presents a stable, continuous, and moderately rough surface morphology, which may support favorable biological interactions and its potential application in soft tissue and periodontal regeneration.

References

1. Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem 2009; 78: 929-958.

2. Ricard-Blum S. The collagen family. Cold Spring Harb Perspect Biol 2011; 3, a004978.

3. Cojocaru C, Budala DG, Virvescu DI, et al. The Role and Involvement of Functional Three-Dimensional Porcine-Derived Collagen Matrix Biomaterials in Periodontal Regeneration: A Comprehensive Review. J. Funct Biomater 2025; 16: 417.

4. Sanz M, Lorenzo R, Aranda JJ, Martin C, Orsini M. Clinical evaluation of a new collagen matrix. J Clin Periodontol 2009; 36: 868-876.

5. Anselme K. Osteoblast adhesion on biomaterials. Biomaterials 2000; 21: 667-681.

6. Bacakova L, Filova, E, Parizek M, Ruml T, Svorcik V. Modulation of cell adhesion. Biotechnol Adv 2011; 29: 739-767.

7. Dalby MJ, Gadegaard N, Oreffo ROC. Harnessing nanotopography. Nat Mater 2014; 13: 558-569.

8. Dufrêne YF. Atomic force microscopy in microbiology. Nat Rev Microbiol 2008; 6: 674-680.

9. Cappella B, Dietler G. Force-distance curves. Surf Sci Rep 1999; 34: 1-104.

10. Fratzl P. Collagen: Structure and mechanics. New York, USA: Springer, 2008.

11. Grant CA, Brockwell DJ, Radford SE, Thomson NH. Effects of hydration on collagen. Biophys J 2009; 97: 2985-2992.

12. Wen J, Wilker JJ. Surface characterization of biomaterials. Langmuir 2011; 27: 12174-12181.

13. Grant CA, Brockwell DJ, Radford, SE, Thomson NH. Effects of hydration on the mechanical response of collagen. Biophys J 2009; 97: 2985-2992.

14. Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem 2009; 78: 929-958.

15. Cappella B, Dietler G. Force-distance curves by atomic force microscopy. Surf Sci Rep 1999 34: 1-104.

16. Ricard-Blum S. The collagen family. Cold Spring Harb Perspect Biol 2011; 3: a004978.

17. Anselme K. Osteoblast adhesion on biomaterials. Biomaterials 2000; 21: 667-681.

18. Butt HJ, Cappella B, Kappl M. Force measurements with the atomic force microscope. Surf Sci Rep 2005; 59: 1-152.

19. García R, Pérez R. Dynamic atomic force microscopy methods. Surf Sci Rep 2002; 47: 197-301.

20. Santos S, Barcons V, Font J, Thomson NH. Stability of AFM imaging in liquids. Nanotechnology 2010; 21: 225710.

21. Xu W, Wang H, Li X. AFM characterization of soft biomaterials in liquid environments. Colloids Surf B 2020; 190: 110948.

22. Zhang Y, Chen X, Li J. Advances in AFM imaging of biological materials. Materials 2021; 14: 3921.

23. Stylianou A, Lekka M, Stylianopoulos T. AFM assessing nanomechanical properties of biological materials. Materials 2018; 11: 186.

24. Parenteau-Bareil R, Gauvin R, Berthod F. Collagen-based biomaterials. Materials 2010; 3: 1863-1887.

25. Ferreira AM, Gentile P, Chiono V, Ciardelli G. Collagen for bone regeneration. Acta Biomater 2012; 8: 3191-3200.

26. Ramshaw JAM. Collagen biomaterials. Biomed Mater 2019; 14: 032001.

27. Zhao X, Huebsch N, Mooney DJ, Suo Z. Hydrogels and soft biomaterials. Nat Rev Mater 2020; 5:32-50.

28. Chaudhuri O. Hydrogels and ECM mechanics. Nat Rev Mol Cell Biol 2017; 18: 689-705.

29. Kim DH, Provenzano PP, Smith CL, Levchenko A. Matrix nanotopography. J Cell Biol. 2012; 197: 351-360.

30. Akhremitchev BB, Walker GC. AFM of biomaterials in liquid. Langmuir 2019; 35: 13967-13978.

31. Virvescu DI, Nicolaiciuc OS, Rotundu G, et al. An Update Regarding the Use of Contemporary Dental Materials in Periodontal Regeneration. Materials 2025; 18: 4278.

32. Chen S, Jones JA, Xu Y, Low HY, Anderson JM, Leong KW. Surface roughness and cell response. Biomaterials 2010; 31:3479-3491.

33. Li M, Liu Q, Jia Z, Xu X. Surface characterization of collagen materials. Colloids Surf B 2019; 173: 531-540.

34. Yang G, Xiao Z, Long H, Ma K, Zhang J, Ren X. Assessment of biomaterial surfaces. Front Bioeng Biotechnol 2018; 6: 135.

35. Dokukin ME, Sokolov I. AFM in liquids and soft materials. Langmuir 2012; 28: 16060-16071.

Additional Files

Published

2026-06-01

Issue

Vol. 130 No. 2 (2026): The Medical-Surgical Journal

Section

DENTAL MEDICINE

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