ARMILLARIA MELLEA: PHENOLIC CONTENT, IN VITRO ANTIOXIDANT AND ANTIHYPERGLYCEMIC EFFECTS

Authors

  • Daniela Elena ZAVASTIN University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi
  • Cornelia MIRCEA University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi
  • Ana Clara APROTOSOAIE University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi
  • Simona GHERMAN University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi
  • Monica HANCIANU University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi
  • Anca MIRON University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi

Abstract

Armillaria mellea, known as honey mushroom, has been used both as food and medicine. Aim: In this work, the ethanolic and hydromethanolic extracts from Armillaria mellea fruiting bodies were investigated for their phenolic content, antioxidant and antihyperglycemic effects (in vitro studies). Material and methods: The total phenolics were quantified using Folin-Ciocalteu assay. The antioxidant activity was evaluated by testing the free radical scavenging capacity, reducing power and ferrous ion chelating ability; in addition, the capacity to inhibit 15-lipoxygenase was also assessed. The antihyperglycemic activity was investigated by α-glucosidase assay. Results: Total phenolic contents of 21.68 ± 0.06 and 5.70 ± 0.28 mg/g were determined in the hydromethanolic and ethanolic extracts, respectively. The hydromethanolic extract showed higher free radical scavenging and reducing abilities (EC50 = 452.6 ± 2.7, 140.57 ± 1.45 and 129.45 ± 0.98 μg/mL in DPPH, ABTS and reducing power assays, respectively). The ethanolic extract proved to be more efficient in the ferrous ion chelation, 15-lipoxygenase and a-glucosidase inhibition assays (EC50 = 67.93 ± 0.35, 290.93 ± 2.05 and 8.54 ± 0.06 μg/mL, respectively). Conclusions: Armillaria mellea extracts showed antioxidant and antihyperglycemic potential in in vitro models and therefore they are promising candidates for the development of dietary supplements and pharmaceutical products.

Author Biographies

  • Daniela Elena ZAVASTIN, University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi

    Faculty of Pharmacy
    Department of Physical Chemistry

  • Cornelia MIRCEA, University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi

    Faculty of Pharmacy
    Department of Pharmaceutical Biochemistry and Clinical Laboratory

  • Ana Clara APROTOSOAIE, University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi

    Faculty of Pharmacy
    Department of Pharmacognosy

  • Simona GHERMAN, University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi

    Faculty of Pharmacy
    Department of Physical Chemistry

  • Monica HANCIANU, University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi

    Faculty of Pharmacy
    Department of Pharmacognosy

  • Anca MIRON, University of Medicine and Pharmacy ”Grigore T. Popa”- Iasi

    Faculty of Pharmacy
    Department of Pharmacognosy

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Additional Files

Published

2015-03-31